Coding

Part:BBa_K1746000

Designed by: Tushar Nichakawade   Group: iGEM15_Cooper_Union   (2015-09-11)

TdT delta1-27 (N-terminal Truncated Variant of TdT)

This DNA sequence is a truncation of full length bovine TdT that was originally designed by Repasky et. all in a Mutational Analysis of Terminal Deoxynucleotidyltransferase Mediated N-Nucleotide Addition in V(D)J Recombination. This deletion was of a putative nuclear localization sequence. This sequence was demonstrated to show 80% catalytic activity relative to full length, untruncated, TdT in 293T cells. Expression of this sequence results in a protein that can be used for 3'-end non-templated synthesis of oligonucleotides.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 76
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1306
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 855


[edit]
Categories
Parameters
None